Review



flow cytometric analysis  (Tree Star Inc)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    Tree Star Inc flow cytometric analysis
    Characterization, cytotoxicity, uptake, and drug release of biomimetic liposomes. (A, B) Mean diameter (A) and Zeta potential (B) of PLip ( n = 3). (C) The representative Cryo-EM image depicted PLip laden with gambogic acid. Scale bar = 100 nm. (D) CCK-8 assay to evaluate the viability of 4T1 cells after various treatments ( n = 3). (E, F) Representative flow cytometry plots depicting apoptosis in tumor cells following various treatments (F), along with a corresponding statistical graph of late apoptosis (E) ( n = 3). (G, H) Observations of cellular uptake of DiD-labeled liposomes under various treatments using fluorescence confocal microscopy (G, scale bar = 20 μm), and flow <t>cytometric</t> quantification (H) ( n = 3). (I) Alterations of CD44 protein expression in tumor cells following HIFU treatment ( n = 3). (J) Comparison of PLip’s drug release profile in high H 2 O 2 conditions versus an H 2 O 2 -free environment ( n = 3). (K, L) Intracellular (K) and extracellular (L) H 2 O 2 levels following various treatments ( n = 3). Data are presented as mean ± SD. Statistical significance was analyzed by one-way ANOVA with a Tukey post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001 vs. indicated; ns, no significance.
    Flow Cytometric Analysis, supplied by Tree Star Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flow cytometric analysis/product/Tree Star Inc
    Average 86 stars, based on 1 article reviews
    flow cytometric analysis - by Bioz Stars, 2026-06
    86/100 stars

    Images

    1) Product Images from "Biomimetic liposomes synergize with high-intensity focused ultrasound to induce ferroptosis in tumors"

    Article Title: Biomimetic liposomes synergize with high-intensity focused ultrasound to induce ferroptosis in tumors

    Journal: Acta Pharmaceutica Sinica. B

    doi: 10.1016/j.apsb.2026.02.024

    Characterization, cytotoxicity, uptake, and drug release of biomimetic liposomes. (A, B) Mean diameter (A) and Zeta potential (B) of PLip ( n = 3). (C) The representative Cryo-EM image depicted PLip laden with gambogic acid. Scale bar = 100 nm. (D) CCK-8 assay to evaluate the viability of 4T1 cells after various treatments ( n = 3). (E, F) Representative flow cytometry plots depicting apoptosis in tumor cells following various treatments (F), along with a corresponding statistical graph of late apoptosis (E) ( n = 3). (G, H) Observations of cellular uptake of DiD-labeled liposomes under various treatments using fluorescence confocal microscopy (G, scale bar = 20 μm), and flow cytometric quantification (H) ( n = 3). (I) Alterations of CD44 protein expression in tumor cells following HIFU treatment ( n = 3). (J) Comparison of PLip’s drug release profile in high H 2 O 2 conditions versus an H 2 O 2 -free environment ( n = 3). (K, L) Intracellular (K) and extracellular (L) H 2 O 2 levels following various treatments ( n = 3). Data are presented as mean ± SD. Statistical significance was analyzed by one-way ANOVA with a Tukey post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001 vs. indicated; ns, no significance.
    Figure Legend Snippet: Characterization, cytotoxicity, uptake, and drug release of biomimetic liposomes. (A, B) Mean diameter (A) and Zeta potential (B) of PLip ( n = 3). (C) The representative Cryo-EM image depicted PLip laden with gambogic acid. Scale bar = 100 nm. (D) CCK-8 assay to evaluate the viability of 4T1 cells after various treatments ( n = 3). (E, F) Representative flow cytometry plots depicting apoptosis in tumor cells following various treatments (F), along with a corresponding statistical graph of late apoptosis (E) ( n = 3). (G, H) Observations of cellular uptake of DiD-labeled liposomes under various treatments using fluorescence confocal microscopy (G, scale bar = 20 μm), and flow cytometric quantification (H) ( n = 3). (I) Alterations of CD44 protein expression in tumor cells following HIFU treatment ( n = 3). (J) Comparison of PLip’s drug release profile in high H 2 O 2 conditions versus an H 2 O 2 -free environment ( n = 3). (K, L) Intracellular (K) and extracellular (L) H 2 O 2 levels following various treatments ( n = 3). Data are presented as mean ± SD. Statistical significance was analyzed by one-way ANOVA with a Tukey post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001 vs. indicated; ns, no significance.

    Techniques Used: Liposomes, Zeta Potential Analyzer, Cryo-EM Sample Prep, CCK-8 Assay, Flow Cytometry, Labeling, Fluorescence, Confocal Microscopy, Expressing, Comparison



    Similar Products

    flow cytometric analysis  (Miltenyi Biotec)
    98
    Miltenyi Biotec flow cytometric analysis
    Flow <t>cytometric</t> analysis of antibody reactivity against LILR-expressing K562 cells. (A) Expression of individual LILRs on transfected K562 cells was confirmed by flow cytometry using the following antibodies: LILRB1, clone GHI/75; LILRB2, clone 42D1; LILRB3, clone MKT5.1; LILRB4, clone ZM4.1; LILRB5, clone 395239; LILRA1, clone 586326; LILRA2, clone 600007; LILRA3, rabbit polyclonal antibody; LILRA4, clone 17G10.2; LILRA5, clone 711828; LILRA6, clone 921330. (B) Representative examples of cross-reactivity (anti-LILRA1 antibody clone 586326), specific reactivity (anti-LILRA2 antibody clone 600007), and weak reactivity (anti-LILRA3 antibody clone 2E9). Blue histograms represent parental K562 cells; red histograms represent LILR-expressing cells.
    Flow Cytometric Analysis, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flow cytometric analysis/product/Miltenyi Biotec
    Average 98 stars, based on 1 article reviews
    flow cytometric analysis - by Bioz Stars, 2026-06
    98/100 stars
    		  Buy from Supplier
    sh800 cell sorter  (Sony Biotechnology)
    99
    Sony Biotechnology sh800 cell sorter
    Flow <t>cytometric</t> analysis of antibody reactivity against LILR-expressing K562 cells. (A) Expression of individual LILRs on transfected K562 cells was confirmed by flow cytometry using the following antibodies: LILRB1, clone GHI/75; LILRB2, clone 42D1; LILRB3, clone MKT5.1; LILRB4, clone ZM4.1; LILRB5, clone 395239; LILRA1, clone 586326; LILRA2, clone 600007; LILRA3, rabbit polyclonal antibody; LILRA4, clone 17G10.2; LILRA5, clone 711828; LILRA6, clone 921330. (B) Representative examples of cross-reactivity (anti-LILRA1 antibody clone 586326), specific reactivity (anti-LILRA2 antibody clone 600007), and weak reactivity (anti-LILRA3 antibody clone 2E9). Blue histograms represent parental K562 cells; red histograms represent LILR-expressing cells.
    Sh800 Cell Sorter, supplied by Sony Biotechnology, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sh800 cell sorter/product/Sony Biotechnology
    Average 99 stars, based on 1 article reviews
    sh800 cell sorter - by Bioz Stars, 2026-06
    99/100 stars
    		  Buy from Supplier
    flow cytometric analysis fluorescent monoclonal antibody kit  (Tongsheng Inc)
    86
    Tongsheng Inc flow cytometric analysis fluorescent monoclonal antibody kit
    Flow <t>cytometric</t> analysis of antibody reactivity against LILR-expressing K562 cells. (A) Expression of individual LILRs on transfected K562 cells was confirmed by flow cytometry using the following antibodies: LILRB1, clone GHI/75; LILRB2, clone 42D1; LILRB3, clone MKT5.1; LILRB4, clone ZM4.1; LILRB5, clone 395239; LILRA1, clone 586326; LILRA2, clone 600007; LILRA3, rabbit polyclonal antibody; LILRA4, clone 17G10.2; LILRA5, clone 711828; LILRA6, clone 921330. (B) Representative examples of cross-reactivity (anti-LILRA1 antibody clone 586326), specific reactivity (anti-LILRA2 antibody clone 600007), and weak reactivity (anti-LILRA3 antibody clone 2E9). Blue histograms represent parental K562 cells; red histograms represent LILR-expressing cells.
    Flow Cytometric Analysis Fluorescent Monoclonal Antibody Kit, supplied by Tongsheng Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flow cytometric analysis fluorescent monoclonal antibody kit/product/Tongsheng Inc
    Average 86 stars, based on 1 article reviews
    flow cytometric analysis fluorescent monoclonal antibody kit - by Bioz Stars, 2026-06
    86/100 stars
    		  Buy from Supplier
    flow cytometric analysis  (Tree Star Inc)
    86
    Tree Star Inc flow cytometric analysis
    Characterization, cytotoxicity, uptake, and drug release of biomimetic liposomes. (A, B) Mean diameter (A) and Zeta potential (B) of PLip ( n = 3). (C) The representative Cryo-EM image depicted PLip laden with gambogic acid. Scale bar = 100 nm. (D) CCK-8 assay to evaluate the viability of 4T1 cells after various treatments ( n = 3). (E, F) Representative flow cytometry plots depicting apoptosis in tumor cells following various treatments (F), along with a corresponding statistical graph of late apoptosis (E) ( n = 3). (G, H) Observations of cellular uptake of DiD-labeled liposomes under various treatments using fluorescence confocal microscopy (G, scale bar = 20 μm), and flow <t>cytometric</t> quantification (H) ( n = 3). (I) Alterations of CD44 protein expression in tumor cells following HIFU treatment ( n = 3). (J) Comparison of PLip’s drug release profile in high H 2 O 2 conditions versus an H 2 O 2 -free environment ( n = 3). (K, L) Intracellular (K) and extracellular (L) H 2 O 2 levels following various treatments ( n = 3). Data are presented as mean ± SD. Statistical significance was analyzed by one-way ANOVA with a Tukey post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001 vs. indicated; ns, no significance.
    Flow Cytometric Analysis, supplied by Tree Star Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flow cytometric analysis/product/Tree Star Inc
    Average 86 stars, based on 1 article reviews
    flow cytometric analysis - by Bioz Stars, 2026-06
    86/100 stars
    		  Buy from Supplier
    firoozabady e rapid flow cytometric analysis  (Galbraith Laboratories Inc)
    86
    Galbraith Laboratories Inc firoozabady e rapid flow cytometric analysis
    Characterization, cytotoxicity, uptake, and drug release of biomimetic liposomes. (A, B) Mean diameter (A) and Zeta potential (B) of PLip ( n = 3). (C) The representative Cryo-EM image depicted PLip laden with gambogic acid. Scale bar = 100 nm. (D) CCK-8 assay to evaluate the viability of 4T1 cells after various treatments ( n = 3). (E, F) Representative flow cytometry plots depicting apoptosis in tumor cells following various treatments (F), along with a corresponding statistical graph of late apoptosis (E) ( n = 3). (G, H) Observations of cellular uptake of DiD-labeled liposomes under various treatments using fluorescence confocal microscopy (G, scale bar = 20 μm), and flow <t>cytometric</t> quantification (H) ( n = 3). (I) Alterations of CD44 protein expression in tumor cells following HIFU treatment ( n = 3). (J) Comparison of PLip’s drug release profile in high H 2 O 2 conditions versus an H 2 O 2 -free environment ( n = 3). (K, L) Intracellular (K) and extracellular (L) H 2 O 2 levels following various treatments ( n = 3). Data are presented as mean ± SD. Statistical significance was analyzed by one-way ANOVA with a Tukey post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001 vs. indicated; ns, no significance.
    Firoozabady E Rapid Flow Cytometric Analysis, supplied by Galbraith Laboratories Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/firoozabady e rapid flow cytometric analysis/product/Galbraith Laboratories Inc
    Average 86 stars, based on 1 article reviews
    firoozabady e rapid flow cytometric analysis - by Bioz Stars, 2026-06
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Flow cytometric analysis of antibody reactivity against LILR-expressing K562 cells. (A) Expression of individual LILRs on transfected K562 cells was confirmed by flow cytometry using the following antibodies: LILRB1, clone GHI/75; LILRB2, clone 42D1; LILRB3, clone MKT5.1; LILRB4, clone ZM4.1; LILRB5, clone 395239; LILRA1, clone 586326; LILRA2, clone 600007; LILRA3, rabbit polyclonal antibody; LILRA4, clone 17G10.2; LILRA5, clone 711828; LILRA6, clone 921330. (B) Representative examples of cross-reactivity (anti-LILRA1 antibody clone 586326), specific reactivity (anti-LILRA2 antibody clone 600007), and weak reactivity (anti-LILRA3 antibody clone 2E9). Blue histograms represent parental K562 cells; red histograms represent LILR-expressing cells.

    Journal: Frontiers in Immunology

    Article Title: Validation of LILR antibody specificities and development of LILRA3-specific antibodies

    doi: 10.3389/fimmu.2026.1729905

    Figure Lengend Snippet: Flow cytometric analysis of antibody reactivity against LILR-expressing K562 cells. (A) Expression of individual LILRs on transfected K562 cells was confirmed by flow cytometry using the following antibodies: LILRB1, clone GHI/75; LILRB2, clone 42D1; LILRB3, clone MKT5.1; LILRB4, clone ZM4.1; LILRB5, clone 395239; LILRA1, clone 586326; LILRA2, clone 600007; LILRA3, rabbit polyclonal antibody; LILRA4, clone 17G10.2; LILRA5, clone 711828; LILRA6, clone 921330. (B) Representative examples of cross-reactivity (anti-LILRA1 antibody clone 586326), specific reactivity (anti-LILRA2 antibody clone 600007), and weak reactivity (anti-LILRA3 antibody clone 2E9). Blue histograms represent parental K562 cells; red histograms represent LILR-expressing cells.

    Article Snippet: Flow cytometric analysis was conducted using the MACSQuant Analyzer 10 (Miltenyi Biotec), and data were analyzed using FlowJo software (BD Biosciences).

    Techniques: Expressing, Transfection, Flow Cytometry

    Characterization, cytotoxicity, uptake, and drug release of biomimetic liposomes. (A, B) Mean diameter (A) and Zeta potential (B) of PLip ( n = 3). (C) The representative Cryo-EM image depicted PLip laden with gambogic acid. Scale bar = 100 nm. (D) CCK-8 assay to evaluate the viability of 4T1 cells after various treatments ( n = 3). (E, F) Representative flow cytometry plots depicting apoptosis in tumor cells following various treatments (F), along with a corresponding statistical graph of late apoptosis (E) ( n = 3). (G, H) Observations of cellular uptake of DiD-labeled liposomes under various treatments using fluorescence confocal microscopy (G, scale bar = 20 μm), and flow cytometric quantification (H) ( n = 3). (I) Alterations of CD44 protein expression in tumor cells following HIFU treatment ( n = 3). (J) Comparison of PLip’s drug release profile in high H 2 O 2 conditions versus an H 2 O 2 -free environment ( n = 3). (K, L) Intracellular (K) and extracellular (L) H 2 O 2 levels following various treatments ( n = 3). Data are presented as mean ± SD. Statistical significance was analyzed by one-way ANOVA with a Tukey post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001 vs. indicated; ns, no significance.

    Journal: Acta Pharmaceutica Sinica. B

    Article Title: Biomimetic liposomes synergize with high-intensity focused ultrasound to induce ferroptosis in tumors

    doi: 10.1016/j.apsb.2026.02.024

    Figure Lengend Snippet: Characterization, cytotoxicity, uptake, and drug release of biomimetic liposomes. (A, B) Mean diameter (A) and Zeta potential (B) of PLip ( n = 3). (C) The representative Cryo-EM image depicted PLip laden with gambogic acid. Scale bar = 100 nm. (D) CCK-8 assay to evaluate the viability of 4T1 cells after various treatments ( n = 3). (E, F) Representative flow cytometry plots depicting apoptosis in tumor cells following various treatments (F), along with a corresponding statistical graph of late apoptosis (E) ( n = 3). (G, H) Observations of cellular uptake of DiD-labeled liposomes under various treatments using fluorescence confocal microscopy (G, scale bar = 20 μm), and flow cytometric quantification (H) ( n = 3). (I) Alterations of CD44 protein expression in tumor cells following HIFU treatment ( n = 3). (J) Comparison of PLip’s drug release profile in high H 2 O 2 conditions versus an H 2 O 2 -free environment ( n = 3). (K, L) Intracellular (K) and extracellular (L) H 2 O 2 levels following various treatments ( n = 3). Data are presented as mean ± SD. Statistical significance was analyzed by one-way ANOVA with a Tukey post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001 vs. indicated; ns, no significance.

    Article Snippet: Subsequently, flow cytometric analysis was performed on the cells using FlowJo v.7.6.5 (Tree Star, Inc., Ashland, USA).

    Techniques: Liposomes, Zeta Potential Analyzer, Cryo-EM Sample Prep, CCK-8 Assay, Flow Cytometry, Labeling, Fluorescence, Confocal Microscopy, Expressing, Comparison